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KMID : 0378019630060080063
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New Medical Journal
1963 Volume.6 No. 8 p.63 ~ p.76
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Studies on the Antigens of Shigella Floxneri
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Abstract
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Strains of Shigella flexneri la and lb obtained from the Communicable Disease Center (International Shigella Center), Atlanta, Georgia, U. S. A. and Niigata Hygiene Laboratory, Japan were studied for their antigenic characteristics by means of agglutinin absorption method. The following results were obtained.
1. Japanese strains Ia and lb showed strong reactions of type specific factor I and were considered to have full amount of this factor. The reactions of group factors were generally weak and the distribution of this factors did not follows the antigenic schema of Ewing.
2. C.D.C. strain la appeared to have only small amounts of type specific factor I, as this strain showed very weak reaction with type specific factor I serum prepared from Japanes strain la. It also revealed negative reaction with that prepared from lb. The type specific factor I of C. D. C. strains Ib was the same as that of Japanse strain 1b, but did not react with la when sera of C. D. C. strain lb was absorbed with some C. D. C. strains.
3. The type specific factor I of la and lb were considered to be composed of two fractions: one which reacted only with their respective subtype serum, and the other which reacted with type sera of both Ia and Ib. Therefore, the author proposed the schema of type specific factor I as follows.
C. D. C. strain Ia I1
Ib 12, 3 .
Japanese strain Ia 11,3 ...
lb 12,3 ...
(Ii and I2 designate the factors which reacted with only their respective subtype serum and I3 the factor which reacted with both subtype sera).
4. The peculiar type factor I1 and 12 were contained in small amounts in the respective subtype strain, and able to be identified by agglutinin absorption reaction. These factor became evident in the strains of being varied.
5. Group factor antigens of C. D. C. strain la were very complicated, and showed strong cross reaction with C. D. C. strains 3a, 3b, 4a and var, Y. The cross reactions could not be explained by the distribution of group factro 3,4 which is the major determinant of subgroup la.
6. The group factor 3, 4 of C. D. C. strain Ib reacted only weakly with the corresponding factor serum, while group factor 6 reacted strongly with i~cra of 3a and 3b which contain this factor abundantly.
7. The process of antigenic changes during the variation of Shigella flexneri la and 1b, and the reacti\ ity and the agglutinin.-binding capacity of this group were discussed. It should be essential to pay special attention for preparing the type specific factor sera.
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KEYWORD
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